Resources for Zebrafish Researchers
ZFIN is a US National Institutes of Health-supported database that collects and disseminates information about zebrafish research. This is the first place to look for information on a specific gene or pathway of interest. ZFIN also connects directly to ZIRC so you can order the fish lines you need.
The Zebrafish International Resource Center (ZIRC) is a searchable collection of live zebrafish lines. It is possible to order mutant or transgenic from ZIRC.
FlipTrap Database is a searchable database of mapped FlipTrap insertional alleles with images and Digital Fish Atlas is in development. Lines are available on request.
Database of insertional mutants that express GFP or Gal4FF in identified loci.
This is currently the most popular “genome engineering” tool for generation of site-directed mutation in the zebrafish genome.
Design: CHOPCHOP is a web tool for selecting the optimum target sites for CRISPR/Cas9- or TALEN-directed mutagenesis.
Design: The CRISPR design tool is a web tool crafted to simplify the process of CRISPR guide selection in an input DNA sequence.
Plasmids: AddGene plasmid repository
The zebrafish genome has been completed and is almost completely assembled and annotated. Refer to these sites for the latest releases of assembly and annotation, as well as powerful search tools:
UCSC Genome Browser
Sanger Center Zebrafish Mutation Project
The Sanger Center has an ongoing project to identify the location of random mutations for use in the long-term project of understanding the function of each gene in the zebrafish genome. The database is searchable by gene name and fish can be ordered through ZIRC.
This website provides the tools and a guide for using Gateway cloning to easily create transgenic zebrafish lines.
The training network on Zebrafish infection models for pharmaceutical screens
The Latin American Zebrafish Network
The European Society for fish models biology and medicine.
National Institute of Genetics (NIG), Japan.
Opportunity for collaboration and supporting travel expenses. Contact Professor Koichi Kawakami.