Hematology

Zebrafish develop system that may improve bone marrow transplant

Zebrafish develop system that may improve bone marrow transplant

Binder, V. (2015). Zebrafish develop system that may improve bone marrow transplant. Boston Children’s Hospital.

 

Nature Cover 23 July 2015

Leonard Zon, MD, Grousbeck Professor of Pediatric Medicine at Harvard Medical School, Investigator at Howard Hughes Medical Institute, Director of the Stem Cell Program, Children’s Hospital Boston, and colleagues have developed a novel competitive marrow transplantation system in adult zebrafish in which engraftment is measured by in vivo fluorescence imaging of the kidney — the adult haematopoietic site.

Using this model to screen for engraftment enhancing activity, the authors identified epoxyeicostrienoic acids, including 11,12-epoxyeicosatrienoic acid (EET), as agents able to enhance engraftment and HSPC specification through the activation of a transcription factor Runx1-mediated expression program. This effect of EET is conserved in mice, indicating possible clinical potential for EET to promote bone marrow transplants.

The findings have been published in the July 23, 2015 issue of Nature. The cover picture of this issue shows two donor fish in red, one in green, and two recipient casper fish with kidneys in red and green to depict chimeric engraftment, as described in their article (photographic work by Vera Binder, MD, Boston Children’s Hospital, graphical work by Ellen van Rooijen, PhD, Boston Children’s Hospital).